Nocardia seriolae Nucleic Acid Detection Kit (Real time PCR-Fluorescent Probe method) Intended use: The kit is used for the epidemiological analysis of Nocardia seriolae in fish viscera tissue samples Principle: In this kit, the specific fragment of N.seriolae is used to design the primer probe probe, which can specifically bind to a SECTION of DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclide activity of Taq enzyme cuts the 5 '-terminal fluorophore from the probe and makes it free in the reaction system. Therefore, the quenched 3 '-end group was separated and the fluorescence was emitted. The fluorescence PCR instrument was used to detect Nocardia seriolae in a completely closed reaction system Main components: Component Specification PCR reaction liquid 24 tubes Positive control 1 tube Negative control 1 tube Note: components in different batches of kits should not be interchangeable. Sample requirements: 1. Sample type Gills, spleen, kidney and other pathological tissues. 2. Sample collection and transportation Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw. 3. Sample preservation It can be stored for 24 hours at 2?~8?, and can be stored for a long time at -20?.

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