Principle of the Test Factor deficiencies are both congenital and acquired. Factor assays are convenient, functional clotting assays that provide a quantitative determination of activity. A dilution of the patient sample is added to the NoFact depleted plasma of the factor of interest and run in a PT (for the extrinsic and common pathway factors) or in an APTT (for the intrinsic factors). The NoFact factor deficient plasma provides the remaining clotting factors while the patient sample is the sole source of the factor of interest. Under these conditions the clot time is inversely proportional to the concentration of the active factor in the patient sample. Factor activity assays are calibrated with a plasma of known concentration of the factor of interest. PlasmaRef ARN is one of a variety of commercially available calibrators or reference plasmas, including nationally and internationally recognized consensus standards, with defined factor activities.

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