Principle of the Test Lupus anticoagulants are antiphospholipid autoantibodies targeted against complexes of proteins and negatively charged phospholipids. Clinically they are associated with auto-immune disease, recurrent fetal loss, and unexplained venous and arterial thrombosis. Circulating anticoagulants are usually detected by the presence of a prolonged clotting time in global coagulation tests which does not correct on mixing patient plasma (1:1) with normal plasma. These tests are not specific, and cannot distinguish between a factor inhibitor, heparin contamination and a true antiphospholipid antibody without further studies. The hallmark characteristic of lupus anticoagulants is their phospholipid dependence; that is, the prolonged clotting time seen with low phospholipid reagents is corrected with high phospholipid reagents. The Dilute Russell Viper Venom time (DRVVT) is a simple one stage clotting test which can be used with carefully matched low and high phospholipid reagents to detect Lupus Anticoagulants with minimal interference from other types of circulating anticoagulants. LupoTek DetecTin VL and LupoTek CorrecTin VL use Vipera lebetina venom rather than Vipera russelli (Russell’s Viper) venom. Vipera lebetina venom, like Russell’s viper venom, will directly activate Factor X without requiring Factor VII. The activated Factor X in conjunction with Factors V, II, calcium ions and phospholipid will generate thrombin which converts fibrinogen to fibrin, producing a clot in the test system. LupoTek DetecTin VL, the low phospholipid reagent, is designed as the screening reagent to detect a prolongation of the clotting time. LupoTek CorrecTin VL is the high phospholipid reagent that neutralizes the LA and corrects the

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